axiovert 200m inverted widefield fluorescence microscope Search Results


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(A.) Wild type HeLa cell and human platelet lysate was run on a 10% SDS-PAGE gel and blotted with GM130 (M.W. = 130 kDa), GalT (M.W. = ~55 kDa plus possible oligomers) and TGN46 (M.W. = predicted 51 kDa, typically runs as a dimer on SDS-PAGE) antibodies to identify cis, trans and trans Golgi network proteins, respectively. GAPDH is shown as a loading control. Asterisks indicate the position of the Golgi proteins. The blots were imaged with an Odyssey Infrared Imaging System (LI-COR Biosciences). (B.) A representative image of wild type HeLa cells showing a classic” stacked, ribbon-like juxtanuclear Golgi structure in which markers to the Golgi cisternae subcompartments cis, GM130 (blue); Trans, GalT (red); and trans Golgi network, TGN46 (green), overlay image map proximal to each other in deconvolved, <t>spinning-disk</t> <t>confocal</t> micrographs (A′–D′).
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(A.) Wild type HeLa cell and human platelet lysate was run on a 10% SDS-PAGE gel and blotted with GM130 (M.W. = 130 kDa), GalT (M.W. = ~55 kDa plus possible oligomers) and TGN46 (M.W. = predicted 51 kDa, typically runs as a dimer on SDS-PAGE) antibodies to identify cis, trans and trans Golgi network proteins, respectively. GAPDH is shown as a loading control. Asterisks indicate the position of the Golgi proteins. The blots were imaged with an Odyssey Infrared Imaging System (LI-COR Biosciences). (B.) A representative image of wild type HeLa cells showing a classic” stacked, ribbon-like juxtanuclear Golgi structure in which markers to the Golgi cisternae subcompartments cis, GM130 (blue); Trans, GalT (red); and trans Golgi network, TGN46 (green), overlay image map proximal to each other in deconvolved, <t>spinning-disk</t> <t>confocal</t> micrographs (A′–D′).
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(A.) Wild type HeLa cell and human platelet lysate was run on a 10% SDS-PAGE gel and blotted with GM130 (M.W. = 130 kDa), GalT (M.W. = ~55 kDa plus possible oligomers) and TGN46 (M.W. = predicted 51 kDa, typically runs as a dimer on SDS-PAGE) antibodies to identify cis, trans and trans Golgi network proteins, respectively. GAPDH is shown as a loading control. Asterisks indicate the position of the Golgi proteins. The blots were imaged with an Odyssey Infrared Imaging System (LI-COR Biosciences). (B.) A representative image of wild type HeLa cells showing a classic” stacked, ribbon-like juxtanuclear Golgi structure in which markers to the Golgi cisternae subcompartments cis, GM130 (blue); Trans, GalT (red); and trans Golgi network, TGN46 (green), overlay image map proximal to each other in deconvolved, <t>spinning-disk</t> <t>confocal</t> micrographs (A′–D′).
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(A.) Wild type HeLa cell and human platelet lysate was run on a 10% SDS-PAGE gel and blotted with GM130 (M.W. = 130 kDa), GalT (M.W. = ~55 kDa plus possible oligomers) and TGN46 (M.W. = predicted 51 kDa, typically runs as a dimer on SDS-PAGE) antibodies to identify cis, trans and trans Golgi network proteins, respectively. GAPDH is shown as a loading control. Asterisks indicate the position of the Golgi proteins. The blots were imaged with an Odyssey Infrared Imaging System (LI-COR Biosciences). (B.) A representative image of wild type HeLa cells showing a classic” stacked, ribbon-like juxtanuclear Golgi structure in which markers to the Golgi cisternae subcompartments cis, GM130 (blue); Trans, GalT (red); and trans Golgi network, TGN46 (green), overlay image map proximal to each other in deconvolved, <t>spinning-disk</t> <t>confocal</t> micrographs (A′–D′).
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(A.) Wild type HeLa cell and human platelet lysate was run on a 10% SDS-PAGE gel and blotted with GM130 (M.W. = 130 kDa), GalT (M.W. = ~55 kDa plus possible oligomers) and TGN46 (M.W. = predicted 51 kDa, typically runs as a dimer on SDS-PAGE) antibodies to identify cis, trans and trans Golgi network proteins, respectively. GAPDH is shown as a loading control. Asterisks indicate the position of the Golgi proteins. The blots were imaged with an Odyssey Infrared Imaging System (LI-COR Biosciences). (B.) A representative image of wild type HeLa cells showing a classic” stacked, ribbon-like juxtanuclear Golgi structure in which markers to the Golgi cisternae subcompartments cis, GM130 (blue); Trans, GalT (red); and trans Golgi network, TGN46 (green), overlay image map proximal to each other in deconvolved, <t>spinning-disk</t> <t>confocal</t> micrographs (A′–D′).
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(A.) Wild type HeLa cell and human platelet lysate was run on a 10% SDS-PAGE gel and blotted with GM130 (M.W. = 130 kDa), GalT (M.W. = ~55 kDa plus possible oligomers) and TGN46 (M.W. = predicted 51 kDa, typically runs as a dimer on SDS-PAGE) antibodies to identify cis, trans and trans Golgi network proteins, respectively. GAPDH is shown as a loading control. Asterisks indicate the position of the Golgi proteins. The blots were imaged with an Odyssey Infrared Imaging System (LI-COR Biosciences). (B.) A representative image of wild type HeLa cells showing a classic” stacked, ribbon-like juxtanuclear Golgi structure in which markers to the Golgi cisternae subcompartments cis, GM130 (blue); Trans, GalT (red); and trans Golgi network, TGN46 (green), overlay image map proximal to each other in deconvolved, <t>spinning-disk</t> <t>confocal</t> micrographs (A′–D′).
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Image Search Results


(A.) Wild type HeLa cell and human platelet lysate was run on a 10% SDS-PAGE gel and blotted with GM130 (M.W. = 130 kDa), GalT (M.W. = ~55 kDa plus possible oligomers) and TGN46 (M.W. = predicted 51 kDa, typically runs as a dimer on SDS-PAGE) antibodies to identify cis, trans and trans Golgi network proteins, respectively. GAPDH is shown as a loading control. Asterisks indicate the position of the Golgi proteins. The blots were imaged with an Odyssey Infrared Imaging System (LI-COR Biosciences). (B.) A representative image of wild type HeLa cells showing a classic” stacked, ribbon-like juxtanuclear Golgi structure in which markers to the Golgi cisternae subcompartments cis, GM130 (blue); Trans, GalT (red); and trans Golgi network, TGN46 (green), overlay image map proximal to each other in deconvolved, spinning-disk confocal micrographs (A′–D′).

Journal: Platelets

Article Title: Golgi proteins in circulating human platelets are distributed across non-stacked, scattered structures

doi: 10.1080/09537104.2016.1235685

Figure Lengend Snippet: (A.) Wild type HeLa cell and human platelet lysate was run on a 10% SDS-PAGE gel and blotted with GM130 (M.W. = 130 kDa), GalT (M.W. = ~55 kDa plus possible oligomers) and TGN46 (M.W. = predicted 51 kDa, typically runs as a dimer on SDS-PAGE) antibodies to identify cis, trans and trans Golgi network proteins, respectively. GAPDH is shown as a loading control. Asterisks indicate the position of the Golgi proteins. The blots were imaged with an Odyssey Infrared Imaging System (LI-COR Biosciences). (B.) A representative image of wild type HeLa cells showing a classic” stacked, ribbon-like juxtanuclear Golgi structure in which markers to the Golgi cisternae subcompartments cis, GM130 (blue); Trans, GalT (red); and trans Golgi network, TGN46 (green), overlay image map proximal to each other in deconvolved, spinning-disk confocal micrographs (A′–D′).

Article Snippet: Spinning-disk confocal, widefield and super resolution (3D SIM) fluorescence microscopy Spinning-disk confocal image stacks were taken with a 63x/1.40 NA using a BD CARVII (BD Bioscience) spinning-disk confocal microscope accessory fitted to the Zeiss 200M inverted microscope as described previously [ 26 ].

Techniques: SDS Page, Imaging